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Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

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Product Name:

Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit

Cat.#:

JL14326

Regulatory Status:
Alternative:

DNMT3A2; M.HsaIIIA; DNA methyltransferase HsaIIIA

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.15-10ng/mL
Sensitivity:
0.03ng/mL
Specificity:
This kit recognizes Human DNMT3A in samples. No significant cross-reactivity or interference between Human DNMT3A and analogues was observed.
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Epigenetics
  • Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit
  • Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit
  • Human DNA Methyltransferase 3A (DNMT3A) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| DNMT3A

DNA (cytosine-5)-methyltransferase 3A (DNMT3A) is an enzyme that catalyzes the transfer of methyl groups to specific CpG structures in DNA, a process called DNA methylation. This enzyme is responsible for de novo DNA methylation. Such function is to be distinguished from maintenance DNA methylation which ensures the fidelity of replication of inherited epigenetic patterns. While de novo DNA methylation modifies the information passed on by the parent to the progeny, it enables key epigenetic modifications essential for processes such as cellular differentiation and embryonic development, transcriptional regulation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 10 5 2.5 1.25 0.625 0.312 0.15 0
OD 2.28 1.82 1.23 0.8 0.53 0.36 0.23 0.08
Corrected OD 2.2 1.74 1.15 0.72 0.45 0.28 0.15 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human DNMT3A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Tissue Homogenates(n=8) 84-101 96
Cell Lysates(n=8) 92-105 102





 

| Linearity

Samples were spiked with high concentrations of Human DNMT3A and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Tissue Homogenates(n=4) Cell Lysates(n=4)
1 : 2 Range (%) 83-95 88-96
Average (%) 91 93
1 : 4 Range (%) 89-103 88-108
Average (%) 94 98







 
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Sample Data
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Sample Data
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Sample Data
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